Data
Data
Altratech’s data demonstrates ‘sample-to-result’ detection of HIV virus from contrived human whole blood samples at ≤1,000 cp/mL, and with further dilution of the extracted RNA. at 500 cp/mL These performance levels are similar to a leading HIV sample-to-result POC device (400 cp/mL LOD) Further LOD reduction is underway.
Fig. 1
‘Sample-to-Result’ capture & detection of HIV (spiked) RNA from human plasma and human whole blood, at five dilutions to 1000 cp/mL.
Fig. 2
‘Sample-to-Result’ capture & detection of HIV (spiked) RNA @1000 cp/mL from 100uL human whole blood, filtered via a commercial finger-pressure membrane filter device5.
Fig. 3
‘Sample-to-Result’ capture & detection of Accurun HIV RNA virions from 200uL bioIVT human plasma. These were 1,000 copies/mL (n=5 chips) contrived HIV samples; some were diluted down to equivalent 500 cp/mL (n=3). Significance levels were p=0.0000, p=0.0000 and p=0.0004 when tested against the negative sample chips.
Fig. 4
Capacitance-vs-HIV1 & HIV2 immuno-assay Antibodies (n=2). The enzyme in a commercially-available HIV antibody test was replaced with a superparamagnetic PNA-coated reporter bead, allowing detection and quantification on specific sensors on our proprietary CMOS silicon chip.